An F-type lectin domain directs the activity of Streptosporangium roseum alpha-l-fucosidase
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چکیده
منابع مشابه
Complete genome sequence of Streptosporangium roseum type strain (NI 9100T)
Streptosporangium roseum Crauch 1955 is the type strain of the species which is the type species of the genus Streptosporangium. The 'pinkish coiled Streptomyces-like organism with a spore case' was isolated from vegetable garden soil in 1955. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first completed genome sequence of...
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The alpha-L-fucosidase from Thermotoga maritima (Tm alpha fuc) was converted into alpha-L-transfucosidase variants by directed evolution. The wild-type enzyme catalyzes oligosaccharide synthesis by transfer of a fucosyl residue from a pNP-fucoside donor to pNP-fucoside (self-condensation) with alpha-(1-->3) regioselectivity or pNP-galactoside (transglycosylation) with alpha-(1-->2) regioselecti...
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Fucosidosis is a newly recognized inborn error of metabolism involving the accumulation o f fucolipids, fucoglycoproteins, a n d fucornucopolysaccharides. T h r e e families h a v e been reported in the literature (1, 2). 1 T h e enzymic defect in fucosidosis has been established b y Van Hoof and Hers (3) as a specific, nearly complete deficiency of the enzyme ~-L-fucosidase. Thusfar, no report...
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The pellet-associated portion of human brain alpha-L-fucosidase (which represents approx. 20% of the homogenate activity) was solubilized with 0.5% (w/v) Triton X-100, characterized with regard to several properties and compared with the corresponding properties of the soluble supernatant-fluid enzyme in an attempt to find a second alpha-L-fucosidase in human brain. The solubilized and soluble ...
متن کاملHuman liver alpha-L-fucosidase. Purification, characterization, and immunochemical studies.
Human liver alpha-L-fucosidase has been purified 6300-fold to apparent homogeneity with 66% yield by a two-step affinity chromatographic procedure utilizing agarose epsilon-aminocaproyl-fucosamine. Isoelectric focusing revealed that all six isoelectric forms of the enzyme were purified. Polyacrylamide gel electrophoresis of the purified alpha-L-fucosidase demonstrated the presence of six bands ...
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ژورنال
عنوان ژورنال: Glycobiology
سال: 2018
ISSN: 1460-2423
DOI: 10.1093/glycob/cwy079